Here is a Nikon TE300 Inverted Fluorescence Phase Contrast Microscope Pred Ti Unit4
Setup for Brightfield with Phase Contrast and Fluorescence studies.
This Nikon TE300 Inverted Fluorescence Phase Contrast Microscope is in good working order with all aspects tested and working fully.
Microscope optics have been cleaned professionally by our technician under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Nikon TE300 Inverted Fluorescence Phase Contrast Microscope passed all tests and was found to be fully functional:
Tested halogen lamp and source: PASSED
Tested mercury lamp and source: PASSED
Tested port splitter: PASSED
Tested mechanical XY stage to limits: PASSED
Tested mechanical focal adjustment: PASSED
Tested condenser and prisms: PASSED
Tested fluorescence light path and filter cubes: PASSED
Tested objectives for clarity: PASSED
Tested eyepieces for clarity: PASSED
Tested connection from computer to camera for live feed: PASSED
Tested all power switches, knobs and light adjustments: PASSED
During testing this Nikon TE300 Inverted Fluorescence Phase Contrast Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Nikon Eclipse TE300 Inverted Microscope
Binocular Tube Head
(2) CFIUW 10x/25 Eyepieces
Prisms / Condenser:
ELWD 0.3 Condenser
Positions: PhL, Ph1, Ph2, A, C
TE-DH100W Illumination Tower
TE-FM Light Path w/ manual shutters
UV-2E/C- EX: 360/40nm DM: 400nm EM: 460/50nm
C/Y/RFP- EX: 436/10x 492/18x 580/20x EM: 465/30m 535/30m 630/60m
G-2A CY3- EX: 535/50nm DM: 575nm EM: 590 LP
LHS-H100P-1 12V 100W Halogen Lamp w/ TE-PS100W control box
LH-M100CB-1 Mercury Lamp w/ HB-10103AF control box
6-position Mechanical Nosepiece
4x/0.13 Plan Fluor PhL DL
10x/0.30 Plan Fluor DIC L
20x/0.75 CFI Plan Apo VC (this is a high quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
Mechanical XY Stage
70 x 55mm Travel
Metal Stage Insert Disc
Photo / Port Splits:
Left and Head Port view split (100 Left/100 Front)
AmScope MU300 CMOS 3.1MP Digital Color Camera
Computer and Software:
Dell Latitude i5 running Windows 10 with AmScope software
Communication and power cables.
Configuration and Setup:
Illumination Type: Brightfield, Fluorescence, Phase Contrast
Objectives: 4x/0.13 Plan Fluor PhL DL, 10x/0.30 Plan Fluor DIC L, 20x/0.75 CFI Plan Apo VC
Light Sources: 12V 100W Halogen Lamp, 100W Mercury Lamp
Stage: Mechanical XY
This Nikon TE300 Inverted Fluorescence Phase Contrast Microscope is currently configured for Brightfield viewing with Fluorescence and Phase Contrast studies.
The MU300 CMOS camera is capable of taking detailed digital images. Dell laptop included for live feed.
With included objectives of 4x, 10x, 20x along side 10x eyepieces the total magnification of the system is 200x.
In this video we give a general overview of the Nikon TE300 Inverted Fluorescence Phase Contrast Microscope.
We first show the entire system powered on, showing the camera, laptop, microscope and both power supplies.
After this view, we show the software version and confirm camera connection and communication on the laptop screen.
After showing the slide image on screen, we move to the Nikon TE300 Inverted Fluorescence Phase Contrast Microscope and give an overview of some of the mechanical controls and it's included accessories.
Then we show mechanical adjustments verified using the image feed from the camera via the Amscope software.
We conclude the video by showing a final view of the entire Nikon TE300 Inverted Fluorescence Phase Contrast Microscope.
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